Semen Analysis and Its Role in Male Fertility Diagnosis
Jan 2025 Author
Health

Semen Analysis and Its Role in Male Fertility Diagnosis

Male fertility involves various aspects that can be both straightforward and complex. One crucial factor is semen and its analysis. Semen itself is a relatively simple component of male fertility—essentially a fluid that contains sperm. When reproductive experts need to examine sperm, they require a semen sample. This makes semen analysis a vital step in diagnosing male fertility issues. With this understanding, some of you may wonder: why is semen analysis of fertility so important in infertility evaluations? That’s precisely what we’ll explore here.

In this blog, we provide you with insight regarding the role of semen analysis in male fertility diagnosis. Along with this, we shall also discuss when a semen analysis is performed, how to prepare for semen analysis and the ideal range for healthy results. But before we discuss this, let’s first discuss what a semen analysis is first. 

What Is Semen Analysis? 

What Is Semen Analysis

In simple terms, a semen analysis is the process through which the fertility specialist tests the overall quality of your sperm and semen. In this process, a lab technician tests a semen sample for a wide range of factors that affect the overall fertility. Now, because of this, many fertility treatments, such as in vitro fertilization IVF and intrauterine insemination (IUI), depend on semen analysis. 

So in other words, the semen analysis includes a detailed sperm analysis that tracks various aspects of sperm, such as sperm count, sperm concentration, and sperm motility. Now, the good news here is that when compared to other forms of tests used in the evaluation of infertile couples, the semen analysis is a globally standardized test. This form of standardized measurement allows for a more accurate calculation of sperm count. 

In addition to this, the semen analysis can also help identify potential issues. These issues include hormonal imbalances and structural abnormalities, which can contribute to infertility. Semen analysis also provides important information for customizing fertility treatments to individual needs. Now, by addressing issues highlighted through semen analysis, you can increase your chances of getting a successful conception. 

When Is Semen Analysis Performed?

Now that you know what semen analysis is, let’s talk about another aspect of semen analysis - when it is performed. So, let’s answer the question: when exactly is the semen analysis performed? Well, some of the key conditions when semen analysis is performed include: 

  • During Infertility Evaluation: One of the first conditions when the semen analysis is performed is during the infertility evaluation. If you have been trying to conceive for over a year without any form of success, then semen analysis is one of the first tests performed to check if you are fertile or not. 
  • After Vasectomy: Another circumstance where semen analysis may be used is after a vasectomy. After a vasectomy, a semen analysis is done to confirm the success of a vasectomy. If the vasectomy is successful, there should not be any forms of sperm in the semen.
  • Vasectomy Reversal: Semen analysis is not just used when conducting a vasectomy but also when reversing the process. If the semen contains an adequate number of sperm after the process, it is considered a success. 
  • Before Fertility Treatments: Alongside vasectomy, semen analysis is also conducted before starting any form of fertility treatment. This includes analysis before treatments such as IVF and IUI, as semen analysis is essential for testing sperm quality and selecting the best course of action. 
  • Unexplained Symptoms: The semen analysis is also conducted if you are experiencing symptoms such as low libido, erectile dysfunction, or hormonal imbalances. Semen analysis is conducted as a part of the broader evaluation of reproductive health. 
  • Post-treatment Follow-up: Another reason as to why semen analysis is conducted is during conditions such as infection, varicocele, or hormonal imbalances. In these conditions, semen analysis is conducted as a part of post-treatment to check improvements in sperm quality. 

These are just some of the key circumstances where sperm analysis is conducted. Besides the ones we mentioned here, there are many other specific circumstances where this analysis may be conducted. Regardless, we hope you understood the general gist of it all. 

Also Read: Freezing Sperm: Why and When You Should Consider It?

Role Of Semen Analysis Test In Male Infertility Diagnosis

Now, after knowing what semen analysis is and when it is performed, let’s finally talk about the key topic of this blog - the role of semen analysis fertility in male infertility diagnosis. So, what is the specific role of semen analysis? Some of the key ones include: 

1. Sperm Count

The primary role of semen analysis is to count the number of sperm. For those unaware, sperm count refers to the process of counting the sperm in a single milliliter of semen. This is an important aspect of examining male fertility, as low sperm count also known as oligospermia, can reduce the chances of conception. Do note that the normal sperm count is generally around 15 million sperm per milliliter of semen. Do note that low sperm can result from a multitude of causes, such as hormonal imbalances, genetic factors, and environmental exposures. This condition can make natural conception more challenging for couples.

2. Sperm Motility

Besides sperm number, semen analysis also measures the ability of sperm to move effectively. You should note that progressive motility is highly important for sperm to travel through the female reproductive tract to fertilize an egg. If your sperm has poor motility, it can hinder the process of fertilization. Poor motility now may be associated with lifestyle-related factors, infection, or the structural abnormality of the sperm. Reduced chances of successful conception are associated with this condition, and treatment together with lifestyle alterations often improves sperm motility and fertility outcome.

3. Sperm Morphology

The second role of semen analysis lies in the assessment of sperm morphology. But what is a sperm with poor morphology? Well, sperm can be considered to have poor morphology if the shape and structures of the sperm are not normal. This can include abnormally shaped heads or defective tails. Assessing the morphology or the shape of sperms is the third role of semen analysis here in male fertility. This is because abnormal sperm shapes can struggle to penetrate and fertilize the egg.

4. Semen Volume

Following the aspect of number, motility, and the morphology of sperm, the next role is regarding the volume of the semen. In this case, the experts examine the volume of the semen. Low semen volume indicates some sort of issue in prostate glands or seminal vesicles if it happens so. In any case, one must have the proper notion that there could be numerous causes associated with a low semen volume. The conventional one's include hormonal imbalance along blockage of the reproductive tract. This condition may affect the delivery of sperm, reducing the chances of successful fertilization.

5. Liquefaction Time

The next role of semen analysis is to examine the liquefaction time. You should note that once the sperm is ejaculated, it forms a gel-like substance that should liquify within 20 to 30 minutes. The failure in this process can be delayed, and this causes an interference effect on the motility of the sperm cells, as well as on its ability to fertilize the egg. Undue delayed liquefaction can indicate the infection or abnormal seminal fluid composition. This may result in reducing the effectiveness of the sperm while in navigating the female reproductive tract.

6. Fructose Level

Along with liquefaction time, semen analysis is also used to measure fructose levels. Now, for those unaware, semen contains many chemicals, such as fructose. This is a chemical that provides energy for the sperm. Low levels of these chemicals in the semen can indicate issues with the seminal vessels or blockages in the reproductive ducts. These low levels can negatively impact how sperm functions. It can also signal hormonal imbalances, especially low testosterone levels. This condition can also impair the vitality of the sperm and reduce the overall fertility potential. 

7. Semen pH

Following the fructose levels, semen analysis is also used to analyze the pH levels of the semen. Checking the pH levels indicates if the semen is acidic or alkaline. Now, while checking for the pH levels of the semen, it should be between 7.2 and 8.0. If the pH level of semen is abnormal it can indicate signs of infections, inflammations, or other reproductive health insurance. This condition also impairs the motility and viability of sperms, thereby decreasing the possibility of fertilization. It may also be related to reproductive ductal blockage or problems in the accessory glands because of an abnormal pH.

8. White Blood Cell

The last function of semen analysis fertility is related to the study of white blood cells present in the semen, known as leukocytospermia. Blood cells present in the semen may indicate infections or inflammation of the reproductive tract. High levels of white blood cells can also harm the sperms and lead to infertility. It can be a consequence of bacterial infection, viral infection, or an autoimmune disease. The oxidative stress caused by excess white blood cells can impair sperm DNA and overall functionality.

These are the key roles of semen analysis fertility in male fertility diagnosis. Besides the key roles we mentioned here, there can be several other roles. Regardless, we hope you got the general gist of it all.

How To Prepare For Semen Analysis?

How To Prepare For Semen Analysis

So, at this point, we have already covered the core topic of our blog. However, there are still a few aspects that we need to go through. One of these things includes how you can prepare for semen analysis. So the question here is - how do you prepare for semen analysis? Some of the key ways include the following:

  • Avoid Ejaculation: You should abstain from all sexual activities, including masturbation, for at least 24 to 72 hours before the test to ensure that the sperm count is at its peak. This is because ejaculation too frequently reduces the sperm count, and abstinence for too long reduces sperm motility.
  • Avoid Alcohol, Caffeine, and Recreational Drugs: When preparing for semen analysis, it is also recommended to avoid alcohol, caffeine, and substances such as cocaine and marijuana for a minimum of two to five days before the test. This is because these substances can negatively affect the quality, quantity, and motility of the sperm.
  • Discontinue Herbal Medications: Another way to prepare for semen analysis is to avoid herbal medications if you are taking any. Depending on the supplements you are taking, they can interfere with sperm production or affect your hormone levels. Do note that you need to consult an expert before deciding to take this action.
  • Avoid Hormonal Medications (if recommended): In addition to herbal medicines, you must also avoid hormonal medications if your expert recommends that you do so. This is because the hormonal medications you are taking may interfere with the sperm count and quality in semen.
  • Discuss All Medications: The final thing you can do to prepare for semen analysis is to discuss all forms of medications with your doctor. This is because some prescriptions and over-the-counter medications such as antibiotics, antifungal drugs, or chemotherapy agents can impact sperm quality and quantity
  • Follow these steps carefully: By doing so, you can be sure of a semen analysis that gives a clear picture of your male reproductive health.

How Is Semen Analysis Carried Out?

Now that we have gone through what semen analysis is, its role in infertility diagnosis, and how one should prepare for semen analysis, let's finally talk over how semen analysis is done. Now, these steps may depend on your individual case. Having said that, here are several general steps to take. The major steps have been listed here:

1. Sample Collection

This begins with semen sample collection. In this step, you are instructed to masturbate and thereafter ejaculate in a sterile container usually in a private room, a clinic, or at home. For this, the experts ask you to practice abstinence for around one to five days before the test to ensure that you have the best sperm volume. They also ask you to thoroughly wash your hands and genital area to avoid contamination before the collection of the sample.

2. Handling the Transport

If the sperm is collected at home, the experts then ask you to keep the semen at body temperature and be delivered to the lab within 30 to 60 minutes of collection. You should always follow the specific instructions provided by the doctors to avoid the contamination or degradation of the semen sample.

3. Laboratory Analysis

In this step, the semen is then analyzed under the microscope using specialized equipment to assess several parameters. There are several forms of laboratory analysis. The key ones include:

  • Macroscopic Analysis:
    • Volume: Normal volume is 1.5–5 mL.
    • Color and Appearance: Typically white or gray; discoloration may indicate issues.
    • Viscosity: Assessed to determine the fluidity of the semen after liquefaction.
    • pH Level: Normal semen pH is slightly alkaline (7.2–8.0).
  • Microscopic Analysis:
    • Sperm Count: Number of sperm per milliliter of semen. A normal range is typically 15 million/mL or more.
    • Motility: Percentage of motile sperm and their movement quality (progressive vs. non-progressive).
    • Morphology: Shape and structure of sperm. Normal morphology means better fertility potential.
    • Vitality: percentage of motile sperm in the sample.
    • Agglutination: Tests for clumping of sperm, which may impact motility.
  • Other Tests:
    • White Blood Cells: Tests for infections or inflammation.
    • Fructose Levels: Assures seminal vesicles are working well.
    • Anti-Sperm Antibodies: Tests for antibodies that may impair sperm function.
    • The result may differ depending on the specificity of the tests.

4. Interpretation of Results

After the tests are carried out, the experts then compare them with the references and values set by WHO. If the tests show abnormal results, then you may have to carry out further tests or repeat the testing since one analysis may not be comprehensive.

5. Recommendations after the Test

This is the last step of semen analysis, and in this process, the doctors will recommend further tests or treatment depending on the results of the test. The recommendations may include hormonal testing, genetic screening, and changes in lifestyle.

Sperm Analysis: Ideal Range for Healthy Results

Now, at this point, you must have a general gist regarding the overall gist of the sperm analysis. But so far, we have not covered the ideal range of various parameters that show that you have healthy semen. The question here is, naturally - what is the ideal range of parameters for healthy results? Following are the various parameters along with their ideal range, which shows a healthy semen.

Parameter

Ideal Range

Semen Volume in (ml)

1.4 to 1.7

Total Sperm count (10^6)

33 to 46

Sperm concentration (10^6/ml)

12 to 16

Total motility (%)

38% to 34%

Progressive Motility (%)

31% to 34%

Vitality (%)

55% to 63%

Sperm Morphology (%)

3% to 4%

 

Seek Expert Guidance From The Hardik IVF Center

Seek Expert Guidance From The Hardik IVF Center

So, before we conclude, you may want to know where you can get the best guidance on semen analysis services in Nepal. Well among many options you can choose from, Hardik IVF and Fertility Center is one of the best options for you. But why seek professional advice through Hardik IVF Center?  Some of the main reasons as to why you should consult Hardik with respect to semen analysis are:

  • We are very careful about the results and strive for accuracy and reliability with the help of our highly skilled team of fertility specialists and laboratory technicians.
  • The center is equipped with advanced technology and high-tech equipment for the purpose of proper analysis and diagnosis.
  • We do not stop at tests; we will also be providing you with customized counseling along with the treatment plan after your result for that major step forward with confidence.
  • As among the most trusted IVF centers in Nepal, hence prides in excellence in both care and support related to fertility at all levels.
  • Issues regarding fertility are the ones that usually best remain secret and hidden. Hence, these needs must be experienced under comfortable, confidential conditions.

Conclusion

When it comes to male reproductive health, there are many tests you can take. One such test is semen analysis. Now, there are many roles of semen analysis fertility. Some of the key roles include assessing sperm count, motility, morphology, volume, liquefaction time, and more. Now, depending on the specifics of the analysis, there are many ways you can prepare for it. That said, most of it revolves around taking abstinence and stopping medications that can affect hormones (if recommended).

In this blog, we provide you with an overview of the various aspects of semen analysis fertility in Nepal. We hope you found this helpful. If you seek further insights into the various aspects of infertility, do check out some of our other blogs. And if you want the best semen analysis services in Nepal, look no further than Hardik IVF and Fertility Center.

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FAQ On Semen Analysis And Its Role In Male Fertility Diagnosis

1. What is a good sperm count for fertility?

A normal sperm count according to WHO would be 15 million/mL, but numbers do not mean much; rather, motility and morphology make up the gamut. And if you suspect it, there's a whole diagnosis that awaits from a physician.

2. What is the best time for a sperm test?

Mornings are usually best since sperm quality can be slightly better after a good night’s rest. Timing matters less than following the preparation guidelines. Just get the sample to the lab within 30–60 minutes for accurate results.

3. How do I prepare for a sperm fertility test?

Abstain from ejaculation for 2–5 days to ensure the best sample. Avoid alcohol, smoking, or stress, and stay hydrated. Also, follow any specific instructions your doctor gives you.

4. Why wait 48 hours for a sperm test?

Waiting 48 hours allows sperm count and quality to replenish after ejaculation. Too short or too long a gap can skew results. It’s about hitting the sweet spot for the most accurate analysis!